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FastÄQ^TM Taq DNA Polymerase

Catalog #: EG-1023

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Description

FastÄQ^TM Taq DNA polymerase is an engineered Taq to achieve rapid PCR without compromising specificity and accuracy. It can extend a kilobase of sequence in 15 seconds and with accuracy 50 times higher than Taq DNA Polymerasey. This ultra-thermostable polymerase can be used for amplifying sequencing libraries.

Source

A recombinant E. coli strain carrying the engineered FastÄQ^TM Taq DNA polymerase gene.

Applications

Routine PCR amplification of up to 20 kb DNA fragment.
Blunt-end PCR cloning.
Sequencing library preparation

Unit Definition

One unit is defined as the amount of enzyme required to incorporate 10 nmoles of dNTPs into acid-insoluble form at 74°C in 30 minutes.

Features

High yield, high sensitivity, ultrafast FastÄQTaq DNA Polymerase
Purity: >99%
Specific Activity: >10,000 U/mg
Stable at 4°C

Components

FastÄQ^TM Taq DNA polymerase - 5 units/µL in 50% glycerol, 20 mM Tris-HCl, pH 7.4, 40 mM NaCl,100 mM KCl, 0.1 mM EDTA, and enzyme stabilizers.
10X Reaction Buffer: 160 mM (NH₄)₂SO₄ , 670 mM Tris-HCl, pH 8.8 (at 25 °C), 15 mM MgCl₂ , 0.1% Tween 20

Storage

FastÄQ^TM Taq DNA polymerase is stable for 2 years when stored in a constant temperature freezer at -20°C.

Shipping

4°C

Note

For research use only

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FastÄQTM Taq DNA Polymerase

Please Choose:

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FastÄQ^TM Taq DNA Polymerase