Turbo TEV protease

Turbo TEV protease is an enhanced form of the wild-type  tobacco etch virus (TEV) Nuclear Inclusion a (NIa) protein,  a cysteine protease that recognizes the cleavage site of E-Xaa-Xaa-Y -Xaa-Q-(G/S) (e.g., ENLYFQG),  and cleaves between Q and G or Q and S.  Turbo TEV is non-autoinactivable,  has robust activity at 4^oC, room temperature or 37  ^oC,  and has much higher sequence specificity than factor Xa, thrombin, or enterokinase.  Turbo TEV protease can be used for removing purification tags such as is 6XHis, GST, MBP from fusion proteins.

Source:  E. coli over-expressing synthetic (enhanced) form of TEV NIa proteinase gene.

Size: 1 mg, 1 ml

Specific Activity: > 10 Units/µg. 1 Unit of Turbo TEV Protease cleaves >85% of 3 µg of control substrate in 1 hour at 30^oC.

Storage: Store at -20^oC.