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Thermolabile Uracil-DNA Glycosylase, UDG

Catalog #: EG-1015


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Description

Thermolabile Uracil-DNA Glycosylase (Thermolabile UDG) removes uracil from single-stranded or double-stranded DNA by hydrolyzing the N-glycosylic bond between the deoxyribose and the base leaving an AP (apurinic or apyrimidinic) site. This enzyme ( 1-10 units) is sensitive to heat and can be completely inactivated by a 10 minute incubation at temperatures above 50°C.

Applications
  • Prevention of Carry-over Contamination in PCR
  • Remove Uracil-base from DNA
Source An E. coli strain that carries an UDG gene from a marine bacterium
Unit Definition 1 unit is defined as the amount of Thermolabile UDG required to release 1nmol of Uracil from dU-containing DNA in one hour at 37°C.
Components
  • Thermolabile UDG: 1,000 units/ml in 50 mM Tris-HCl, 50 mM NaCl, 1 mM DTT, 0.1 mM EDTA, 50% glycerol, pH 7.5 @ 25°C
  • 10X Reaction Buffer: 700 mM Tris-HCl, 100 mM NaCl, 10 mM EDTA, 1 mg/mL BSA, pH 8.0 @ 25°C
Quality Control

The absence of single and double-strand exonuclease, and endodeoxyribonucleases is confirmed by appropriate quality tests

Storage Condition

-20 °C


     
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