Thermolabile Uracil-DNA Glycosylase, UDG

Thermolabile Uracil-DNA Glycosylase (Thermolabile UDG) removes uracil from single-stranded or double-stranded DNA by hydrolyzing the N-glycosylic bond between the deoxyribose and the base leaving an AP (apurinic or apyrimidinic) site. This enzyme ( 1-10 units) is sensitive to heat and can be completely inactivated by a 10 minute incubation at temperatures above 50°C.

• Prevention of Carry-over Contamination in PCR
• Remove Uracil-base from DNA

• Thermolabile UDG: 1,000 units/ml in 50 mM Tris-HCl, 50 mM NaCl, 1 mM DTT, 0.1 mM EDTA, 50% glycerol, pH 7.5 @ 25°C
• 10X Reaction Buffer: 700 mM Tris-HCl, 100 mM NaCl, 10 mM EDTA, 1 mg/mL BSA, pH 8.0 @ 25°C

The absence of single and double-strand exonuclease, and endodeoxyribonucleases is confirmed by appropriate quality tests

-20 °C