Human apurinic/apyrimidinic endonuclease, APE 1

Human apurinic/apyrimidinic (AP) endonuclease, APE 1, also known as HAP 1 or Ref-1, is a homolog of Escherichia coli exonuclease III. APE1 acts as an AP lyase by hydrolyzing the phosphodiester backbone at the 5′ end of an apurinic (AP) site, generating a single-strand DNA break in the DNA duplex and leaving 3′-hydroxyl and 5′-deoxyribose phosphate termini. It is reported that APE 1 may also exhibit weak DNA 3′-diesterase, 3′ to 5′ exonuclease and RNase H activities.

• Single cell gel electrophoresis (Comet assay)
• Alkaline elution
• Alkaline unwinding
• Modified nick translation

• APE 1: 10,000 units/ml in 10 mM Tris-HCl, pH 8.0 @ 25°C, 50 mM NaCl, 1 mM DTT, 0.05 mM EDTA, 200 μg/ml BSA, 50% Glycerol
  
• 10X Reaction Buffer: 200 mM Tris-Acetate, 500 mM Potassium Acetate, pH 7.9 @ 25°C, 10 mM Dithiothreitol, 100 mM Magnesium Acetate.

-20 °C