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Wilm’s Tumor Suppressor 1 (-KTS)
Catalog #:
RP-111
$340.00
Qty:
Product Name
Wilm’s Tumor Suppressor 1 (-KTS)
Size
5,000 U
Description
WT-1, the product of Wilms’ tumor suppressor gene Wt1, is a nuclear protein with structural motifs characteristic of transcription factors, including four C-terminal zinc fingers. While different pre-mRNA processing could result in 16 isoforms of the protein, inclusion or exclusion of exon 5 and the three amino acids (KTS) between zinc fingers 3 and 4 largely affects the activity of WT1 protein (1-3). Such a complex post-transcriptional regulation, particularly in splicing, may represent a major regulatory mechanism for tumorigenesis of the Wilms’ tumor. WT1 (-KTS) appears to have different binding affinity to both DNA and RNA comparing to the +KTS form (4, 5).
The N-terminal Flag-tagged WT-1 protein (residue 1-446, including exon 5 but without KTS) was expressed in a baculovirus system and purified by affinity and FPLC chromatography.
Applications
in vitro
function studies including
transcription, DNA or RNA binding assays
protein-protein interaction assay
cell growth and proliferation assays
Source
Baculovirus-insect cell
Fusion Tag
6X His
Purification Method
FPLC
Unit Definition (Activity)
1 unit equals to 1 nanogram of protein. 2-10 units are sufficient for a gel mobility shift assay in a 20µl reaction. 50-200 units are required for a reconstituted transcription assay and 100 units are sufficient for a protein-protein interaction assay.
Purity
~ 95% as determined by SDS-PAGE
Gene ID
7490
Accession #
NM_000378
Gene Name
Wilms tumor 1
Gene Symbol
WT-1
Gene Synonyms
GUD; AWT1; WAGR; WT33; NPHS4; WIT-2; EWS-WT1
MW
55.7 kDa
Protein Sequence
1 MQDPASTCVP EPASQHTLRS GPGCLQQPEQ QGVRDPGGIW AKLGAAEASA ERLQGRRSRG
61 ASGSEPQQMG SDVRDLNALL PAVPSLGGGG GCALPVSGAA QWAPVLDFAP PGASAYGSLG
121 GPAPPPAPPP PPPPPPHSFI KQEPSWGGAE PHEEQCLSAF TVHFSGQFTG TAGACRYGPF
181 GPPPPSQASS GQARMFPNAP YLPSCLESQP AIRNQGYSTV TFDGTPSYGH TPSHHAAQFP
241 NHSFKHEDPM GQQGSLGEQQ YSVPPPVYGC HTPTDSCTGS QALLLRTPYS SDNLYQMTSQ
301 LECMTWNQMN LGATLKGVAA GSSSSVKWTE GQSNHSTGYE SDNHTTPILC GAQYRIHTHG
361 VFRGIQDVRR VPGVAPTLVR SASETSEKRP FMCAYPGCNK RYFKLSHLQM HSRKHTGEKP
421 YQCDFKDCER RFSRSDQLKR HQRRHTGVKP FQCKTCQRKF SRSDHLKTHT RTHTGEKPFS
481 CRWPSCQKKF ARSDELVRHH NMHQRNMTKL QLAL
Storage Buffer
20 mM Tris-Cl (pH 8.0), 20% Glycerol, 100 mM KCl, 1 mM DTT and 0.2 mM EDTA
Storage
-20 to -80 °C.
Shipping
4°C or dry ice
References
1. Haber, DA. et al., (1991) Proc. Natl. Acad. Sci. USA 88, 9618-9622; (1993) Science 262, 2057-2059
2. Kreidberg, JA. et al., (1993) Cell 74, 679-691
3. Larsson, SH. et al., (1995) Cell 81, 391-401
4. Little, M. et al., (1997) Hum. Mutat. 9, 209-225
5. Englert, C. et al., (1995) EMBO J. 14, 4662-4675
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