Wilm’s Tumor Suppressor 1 (-KTS)

Catalog #: RP-111

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Product Name	Wilm’s Tumor Suppressor 1 (-KTS)
Size	5,000 U
Description	WT-1, the product of Wilms’ tumor suppressor gene Wt1, is a nuclear protein with structural motifs characteristic of transcription factors, including four C-terminal zinc fingers. While different pre-mRNA processing could result in 16 isoforms of the protein, inclusion or exclusion of exon 5 and the three amino acids (KTS) between zinc fingers 3 and 4 largely affects the activity of WT1 protein (1-3). Such a complex post-transcriptional regulation, particularly in splicing, may represent a major regulatory mechanism for tumorigenesis of the Wilms’ tumor. WT1 (-KTS) appears to have different binding affinity to both DNA and RNA comparing to the +KTS form (4, 5). The N-terminal Flag-tagged WT-1 protein (residue 1-446, including exon 5 but without KTS) was expressed in a baculovirus system and purified by affinity and FPLC chromatography.
Applications	in vitro function studies including transcription, DNA or RNA binding assays protein-protein interaction assay cell growth and proliferation assays
Source	Baculovirus-insect cell
Fusion Tag	6X His
Purification Method	FPLC
Unit Definition (Activity)	1 unit equals to 1 nanogram of protein. 2-10 units are sufficient for a gel mobility shift assay in a 20µl reaction. 50-200 units are required for a reconstituted transcription assay and 100 units are sufficient for a protein-protein interaction assay.
Purity	~ 95% as determined by SDS-PAGE
Gene ID	7490
Accession #	NM_000378
Gene Name	Wilms tumor 1
Gene Symbol	WT-1
Gene Synonyms	GUD; AWT1; WAGR; WT33; NPHS4; WIT-2; EWS-WT1
MW	55.7 kDa
Protein Sequence	1 MQDPASTCVP EPASQHTLRS GPGCLQQPEQ QGVRDPGGIW AKLGAAEASA ERLQGRRSRG 61 ASGSEPQQMG SDVRDLNALL PAVPSLGGGG GCALPVSGAA QWAPVLDFAP PGASAYGSLG 121 GPAPPPAPPP PPPPPPHSFI KQEPSWGGAE PHEEQCLSAF TVHFSGQFTG TAGACRYGPF 181 GPPPPSQASS GQARMFPNAP YLPSCLESQP AIRNQGYSTV TFDGTPSYGH TPSHHAAQFP 241 NHSFKHEDPM GQQGSLGEQQ YSVPPPVYGC HTPTDSCTGS QALLLRTPYS SDNLYQMTSQ 301 LECMTWNQMN LGATLKGVAA GSSSSVKWTE GQSNHSTGYE SDNHTTPILC GAQYRIHTHG 361 VFRGIQDVRR VPGVAPTLVR SASETSEKRP FMCAYPGCNK RYFKLSHLQM HSRKHTGEKP 421 YQCDFKDCER RFSRSDQLKR HQRRHTGVKP FQCKTCQRKF SRSDHLKTHT RTHTGEKPFS 481 CRWPSCQKKF ARSDELVRHH NMHQRNMTKL QLAL
Storage Buffer	20 mM Tris-Cl (pH 8.0), 20% Glycerol, 100 mM KCl, 1 mM DTT and 0.2 mM EDTA
Storage	-20 to -80 °C.
Shipping	4°C or dry ice
References	1. Haber, DA. et al., (1991) Proc. Natl. Acad. Sci. USA 88, 9618-9622; (1993) Science 262, 2057-2059 2. Kreidberg, JA. et al., (1993) Cell 74, 679-691 3. Larsson, SH. et al., (1995) Cell 81, 391-401 4. Little, M. et al., (1997) Hum. Mutat. 9, 209-225 5. Englert, C. et al., (1995) EMBO J. 14, 4662-4675

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