TNF-alpha (Low Endotoxin) [RP-124] - $220.00 : Excellgen, Recombinant Enzymes

Catalog #: RP-124

$220.00

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Product Name	TNF-alpha (Low Endotoxin )
Size	10 µg
Description	Tumor necrosis factor alpha (TNFα or TNFSF2) is produced by many different cell types including activated monocytes, fibroblasts, endothelial cells, macrophages, T-cells, B-lymphocytes, granulocytes, smooth muscle cells, eosinophilis, chondrocytes and other cell types (1). It functions as a cytokine involved in cell proliferation, differentiation, apoptosis, lipid metabolism and coagulation (2, 3). High level of TNFα leads to the development of inflammatory responses that are hallmarks of many diseases including autoimmune diseases, insulin resistance and cancer (4, 5). The active form of TNFa (18 kDa) is generated by the cleavage of an Ala-Val bond between residues 76-77 of the precursor protein (6).
Applications	cytotoxicity assays and other cell assays
Source	E. coli
Fusion Tag	6X His
Purification Method	FPLC
Unit Definition (Activity)	1 unit equals to 1 nanogram of purified protein.
Purity	~ 95% as determined by SDS-PAGE
Gene ID	7124
Accession #	NM_000594
Gene Name	tumor necrosis factor
Gene Symbol	TNF
Gene Synonyms	DIF; TNFA; TNFSF2; TNF-alpha
MW	21 kDa
Protein Sequence	VRSSSRTPSD KPVAHVVANP QAEGQLQWLN RRANALLANG VELRDNQLVV PSEGLYLIYS QVLFKGQGCP STHVLLTHTI SRIAVSYQTK VNLLSAIKSP CQRETPEGAE AKPWYEPIYL GGVFQLEKGD RLSAEINRPD YLDFAESGQV YFGIIAL
Storage Buffer	20mM Tris-HCl pH7.9,100mM NaCl, 0.2mM EDTA, 1mM DTT and 20% glycerol
Storage	-20 to -80 °C.
Shipping	4°C or dry ice
References	1. Locksley, R. et al., (2001) Cell 104, 487-501 2. Mukhopadhyay, S. et al., (2006) Respiratory Res. 7, 1-9 3. Aggarwal, B. (2003) Nat. Rev. Immunol. 3, 745-756 4. Walczak, H. et al., (1999) Nat. Med. 5, 157-163 5. Lam, J. et al., (2000) J. Clin. Invest. 106, 1481-1488 6. Mcgeehan, G. et al., (1994) Nature 370, 558-561

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