HIF-1a NTAD (Hypoxia-Inducible Factor 1 alpha N-Terminal Activation Domain (530-698)

Catalog #: RP-120

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Product Name	HIF-1a NTAD (Hypoxia-Inducible Factor 1 alpha N-Terminal Activation Domain (530-698)
Size	10,000 U
Description	Hypoxia-inducible factor-1 (HIF1) is a transcription factor found in mammalian cells cultured under reduced oxygen tension that plays an essential role in cellular and systemic homeostatic responses to hypoxia. HIF1 is a heterodimer composed of an alpha subunit and a beta subunit. The beta subunit has been identified as the aryl hydrocarbon receptor nuclear translocator (ARNT). This gene encodes the alpha subunit of HIF-1. HIF-1 alpha contains two transactivation domains located between amino acids 531 and 826. Overexpression of a natural antisense transcript (aHIF) of this gene has been shown to be associated with nonpapillary renal carcinomas. Two alternative transcripts encoding different isoforms have been identified. Specific disruption of the HIF-1 pathway is important for exploring its role in tumor biology and developing more efficient weapons to treat cancer (1). HIF-1alpha is a master regulator of the hypoxic response, and its proangiogenic activities include, but are not limited to, regulation of vascular endothelial growth factor (VEGF) (2). HIF-1alpha protein expression often seen in invasive breast cancer (3). Recent data demonstrates that HIF-1alpha knockdown reduces tumorigenicity of MCF-7 cells and suggest a promising combination of both anti-HIF-1 strategy and traditional chemotherapy to improve cancer treatment (4)
Applications	protein-protein interaction assays in vitro transcription assays cell growth assays
Source	E. coli
Fusion Tag	6X His
Purification Method	FPLC
Unit Definition (Activity)	1 unit equals to 1 nanogram of purified protein
Purity	~ 95% as determined by SDS-PAGE
Gene ID	3091
Accession #	NM_001530
Gene Name	hypoxia inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor)
Gene Symbol	HIF1A
Gene Synonyms	HIF1; MOP1; PASD8; HIF-1A; bHLHe78; HIF-1alpha; HIF1-ALPHA
MW	19.8 kDa
Protein Sequence	EFKLELVEKL FAEDTEAKNP FSTQDTDLDL EMLAPYIPMD DDFQLRSFDQ LSPLESSSAS PESASPQSTV TVFQQTQIQE PTANATTTTA TTDELKTVTK DRMEDIKILI ASPSPTHIHK ETTSATSSPY RDTQSRTASP NRAGKGVIEQ TEKSHPRSPN VLSVALSQRT T
Storage Buffer	20 mM Tris-Cl (pH 8.0), 20% Glycerol, 100 mM KCl, 1 mM DTT and 0.2 mM EDTA
Storage	-20 to -80 °C.
Shipping	4°C or dry ice
References	1. Semenza,G.L. et al., (1994) J. Biol. Chem. 269 (38), 23757-23763 2. Corley,K.M. et al., (2005) J. Cell. Biochem. 96 (5), 971-985 3. Vleugel,M.M., et al., (2005) Cancer Genet. Cytogenet. 163 (2), 168-172 4. Li,J., et al. (2006) Biochem. Biophys. Res. Commun. 342 (4), 1341-1351

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