Product Name: WT-1 (+KTS)
Product Type: Recombinant protein, His tagged.
Gene Name: WT1
Gene Synonym: WT1
Source: Baculovirus-insect cell
Purification Methods: FPLC
Purity: >90% by SDS-PAGE analysis.
Size: 5,000U
Description:
WT-1, the product of Wilms’ tumor suppressor gene Wt1, is a nuclear protein with structural motifs characteristic of transcription factors, including four C-terminal zinc fingers. While different pre-mRNA processing could result in 16 isoforms of the protein, the inclusion or exclusion of exon 5 and three amino acids (KTS) between zinc fingers 3 and 4 largely affects the activity of the WT1 protein (1-3). Such a complex post-transcriptional regulation, particularly in splicing, may represent a major regulatory mechanism for tumorigenesis of the Wilms’ tumor. With the inclusion of exon 5, WT1 (KTS+) binds to both DNA and RNA and is RNase but not DNase sensitive (4). This form also co-localizes with splicing factors in a speckled nuclear particle, suggesting that the WT1 protein may function as both a transcription factor and a splicing regulator (5, 6).
Applications:
1) in vitro function studies including transcription, DNA or RNA binding assays
2) protein-protein interaction assay
3) cell growth and proliferation assays.
Unit definition: 1 unit equals 1 nanogram of purified protein. 1 unit is the amount sufficient for a gel mobility shift assay in a 20 µl reaction. 50 units are sufficient for reconstituted transcription assay and 100 units are sufficient for a protein-protein interaction assay.
1x Dilution buffer: 20mM Tris-HCl pH7.9,100mM NaCl, 0.2mM EDTA, 1mM DTT and 20% glycerol.
References:
1. Haber, DA. et al., (1991) Proc. Natl. Acad. Sci. USA 88, 9618-9622; (1993) Science 262, 2057-2059
2. Kreidberg, JA. et al., (1993) Cell 74, 679-691
3. Larsson, SH. et al., (1995) Cell 81, 391-401
4. Caricasole, A., et al., (1996) Proc. Natl. Acad. Sci. USA, 93, 7562-7566
5. Little, M. et al., (1997) Hum. Mutat. 9, 209-225
6. Englert, C. et al., (1995) EMBO J. 14, 4662-4675
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