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Flag Tag Rabbit Polyclonal Antibody
Catalog #:
EG-275
$168.00
Qty:
Product Name
Flag Tag Rabbit Polyclonal Antibody
Size
100 µg
Overview
The DYKDDDDK peptide (Flag-tag) is a small component of an epitope which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. It has been used extensively as a general epitope tag in expression vectors. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.A Flag-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a Flag-tag to this protein allows one to follow the protein with an antibody against the Flag sequence. This Flag tag polyclonal antibody can be used to detect fusion proteins.with a C-terminal, internal, or N-terminal Flag tag (DYKDDDDK)
Concentration
~ 1 mg/ml
Formulation
20 mM PBS, pH 7.4, 50% glycerol, 0.2% BSA
Usage
WB: 1:5000
QC
Shipping
Blue ice
Storage
-20 oC or 4 oC. Stable for 1 year at -20 oC
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